Genome-wide identification of petunia C2H2 zinc finger family genes and their potential roles in floral volatile benzenoids/phenylpropanoids metabolism.

Jiahong Chen, Xiaomeng Liu, Lingli Jiang, Hongsheng Qing, Jieyu Qian, Zixi Li, Chao Zhang, Jianxin Fu

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Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Jiahong Chen *Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Xiaomeng Liu *Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Lingli JiangZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Hongsheng QingZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Jieyu QianZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Zixi LiZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.

Chao ZhangZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China. zhangc@zafu.edu.cn.

Jianxin FuZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration On Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China. fujx@zafu.edu.cn.

Funding

College Students Research Training Program of Zhejiang Agriculture and Forestry University S202210341087National Natural Science Foundation of China 32002077

6 · The paper itself

Abstract

MAIN

conclusionPhC2H2-ZFP45 may act as a transcriptional activator to up-regulate the expression of PhPAL2 and participate in the formation of floral fragrance. C2H2-zinc finger proteins (C2H2-ZFPs) are involved in the regulation of plant development and stress resistance. However, there are few studies on the effect of C2H2-ZFPs on the regulation of floral fragrance. Petunia hybrida has become an ideal model plant for studying floral volatile benzenoids/phenylpropanoids (FVBP) metabolism. To gain insight into the participation of C2H2-ZFPs in the regulation of floral fragrance in petunia, we performed a genome-wide identification and characterization of C2H2-ZFP genes. A total of 96 C2H2-ZFP genes were identified from the genome of Petunia axillaris, one wild parent of P. hybrida, and their gene structure, conserved motif, phylogenetic relationship, cis-acting elements were analyzed. The length and intron-exon organization in P. axillaris C2H2-ZFP genes were highly heterogeneous. The C2H2 domain was conserved in all C2H2-ZFPs, while the EAR domain was present in 33 C2H2-ZFPs. The P. axillaris C2H2-ZFP gene family was classified into four clades, and clade D contained 51 members. Most petunia C2H2-ZFP genes contained light stress response elements and hormone-related elements. 67 assembled sequences according to reported P. hybrida 'Mitchell' corolla RNA sequencing data could be mapped to the C2H2-ZFP genes of Petunia axillaris. The spatiotemporal expression patterns of PhC2H2-ZFP8 and PhC2H2-ZFP45 well correlated with the developmental and tissue-specific patterns of petunia floral scent formation and emission, suggesting that these genes might be involved in the regulation of FVBP metabolism. Through yeast one hybrid and dual luciferase assay, PhC2H2-ZFP45 was further confirmed to upregulate the expression of PhPAL2. This study will serve as a molecular basis for further exploring the role of PhC2H2-ZFPs in floral scent regulation.

Indexed as

CYS2-HIS2 Zinc FingersFlowersPetuniaPlant ProteinsVolatile Organic CompoundsGene Expression Regulation, PlantGenes, PlantGenome, PlantMultigene FamilyPhylogenyPlant ProteinsVolatile Organic CompoundsC2H2-ZFPExpression patternFlower scentPetunia hybridPhylogenetic analysis

Identifiers

PMID41483185

DOI10.1007/s00425-025-04904-w

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