Evidence map›Paper›PMID 41482385›Full record

ArticleIn vivo (Athens, Greece)

N-myristoyltransferase 1-mediated Src Myristoylation Promotes Non-receptor Tyrosine Kinase Pathways in Oral Squamous Cell Carcinoma.

Takuma Komiyama, Goro Sugiyama, Hanae Kamohara, Takahiro Fujinaga, Wataru Kumamaru, Masafumi Moriyama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Takuma KomiyamaSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Goro SugiyamaSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan gs5603@dent.kyushu-u.ac.jp.
Hanae KamoharaSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Takahiro FujinagaSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Wataru KumamaruSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Masafumi MoriyamaSection of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND

aimN-myristoyltransferase 1 (NMT1), which myristoylates Src, is highly expressed in oral squamous cell carcinoma (OSCC). Although targeted therapies against epidermal growth factor receptor exist, their use is limited by resistance and toxicity, and NMT1-Src interactions remain unexplored. Herein, we aimed to evaluate the role of NMT1-mediated Src myristoylation in the malignant potential of OSCC. MATERIALS AND

methodsMyristoylation and the expression of NMT1 in OSCC were assessed using click chemistry and immunocytochemistry. RNA-seq and enrichment analyses were performed to compare OSCC cells with or without NMT1-siRNA treatment. Src activity was determined by measuring Src phosphorylation via western blotting. Expression and binding of NMT1 and Src were analyzed via immunoprecipitation using specific antibodies. HSC-2, HSC-3, WK2, and WK3F derived from human OSCC

resultsNMT1 and Src expression was detected in all OSCC cell lines. RNA-seq analysis of SAS cells transfected with NMT1-siRNA revealed decreased expression of genes related to cell adhesion and angiogenesis. WK3F cells, which exhibit high malignancy, showed markedly higher NMT1 expression than other cell lines. Immunocytochemistry showed that Src membrane localization was reduced in all of the OSCC cell lines with

conclusionNMT1 promotes OSCC malignancy by mediating Src myristoylation.

Indexed as

AcyltransferasesCarcinoma, Squamous CellMouth Neoplasmssrc-Family KinasesCell Line, TumorGene Expression Regulation, NeoplasticHumansMyristic AcidPhosphorylationSignal TransductionAcyltransferasesglycylpeptide N-tetradecanoyltransferaseMyristic Acidsrc-Family Kinasesintrinsic activationmyristoylationNMT1non-receptor tyrosine kinaseoral squamous cell carcinomaSrc

Identifiers

PMID41482385
PMCPMC12764184

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.