Evidence map›Paper›PMID 41482354›Full record

ArticleCancer genomics & proteomics

Exosomes Transfer ST6GAL1-mediated Therapeutic Resistance in Rectal Cancer Cells.

Jeremie M P Lever, Regina K Irwin, Vivek Somasundaram, Yuwei Song, Chandler M McLeod, Mary Smithson, Gregory Williams, Zechen Chong, Karin M Hardiman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer genomics & proteomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jeremie M P Lever *Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Regina K Irwin *Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Vivek SomasundaramDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Yuwei SongDepartment of Biomedical Informatics and Data Science, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Chandler M McLeodDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Mary SmithsonDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Gregory WilliamsDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Zechen ChongDepartment of Biomedical Informatics and Data Science, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
Karin M HardimanDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A.; khardiman@uabmc.edu.

Funding

XRAY CRYSTALLOGRAPHYP30CA013148 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Robert S Welner · 1985 to 2026
$165.9M
A novel, transferable sialylation-mediated mechanism of chemoradioresistance in GI cancerR01CA265981 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Karin Marie Hardiman · 2022 to 2026
$1.9M
NCI NIH HHS P30 CA013148NCI NIH HHS R01 CA265981
6 · The paper itself

Abstract

BACKGROUND/

aimLocally advanced Rectal cancer (RC) is treated with neoadjuvant chemoradiation, but treatment resistance is common. We have previously shown that ST6GAL1 causes RC chemoradiation resistance. Exosomes are small particles that can transfer proteins between cells. We hypothesized that exosomes transfer ST6GAL1 between RC cells, spreading treatment resistance. MATERIALS AND

methodsWe characterized exosomes isolated from multiple ST6GAL1-expressing colorectal cancer (CRC) cell lines. Treatment response was assessed in ST6GAL1-knockdown (KD) cells treated with these exosomes. Single cell RNA sequencing (scRNA-seq) and flow cytometry for surface sialylation were performed on CRC organoids to compare presence of ST6GAL1 RNA and protein activity.

resultsExosomes from multiple CRC cell lines contained ST6GAL1 protein and ST6GAL1 KD cells treated with these exosomes demonstrated transfer of ST6GAL1 with increase in protein in treated cells, but not mRNA, and the protein localized to the Golgi complex. In addition, treated cells demonstrated increased resistance to chemoradiation-induced apoptosis (

conclusionThese findings demonstrate that ST6GAL1-containing rectal cancer exosomes transfer ST6GAL1 between cells causing treatment resistance.

Indexed as

Antigens, CDDrug Resistance, NeoplasmExosomesRectal NeoplasmsSialyltransferasesApoptosisbeta-D-Galactoside alpha 2-6-SialyltransferaseCell Line, TumorHumansAntigens, CDbeta-D-Galactoside alpha 2-6-SialyltransferaseSialyltransferasesST6GAL1 protein, humanbiomarkerexosomesRectal adenocarcinomasialylationST6GAL1

Identifiers

PMID41482354
PMCPMC12758667

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.