Evidence map›Paper›PMID 41482132›Full record

ArticleBioorganic & medicinal chemistry letters2026

Second generation PSMA-targeted turn-on probe for imaging cargo release in prostate cancer cells.

Nooshin Mesbahi, Brenna C McAllister, Hosog Yoon, Aaron T Hendricksen, Melody D Fulton, Leslie A Caromile, Clifford E Berkman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic & medicinal chemistry letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nooshin MesbahiWashington State University, Department of Chemistry, Pullman, WA 99164-4630., United States.
Brenna C McAllisterUniversity of Connecticut, Center for Vascular Biology, Farmington, CT 06030-3501, United States.
Hosog YoonWashington State University, Department of Chemistry, Pullman, WA 99164-4630., United States.
Aaron T HendricksenWashington State University, Department of Chemistry, Pullman, WA 99164-4630., United States.
Melody D FultonWashington State University, Department of Chemistry, Pullman, WA 99164-4630., United States.
Leslie A CaromileUniversity of Connecticut, Center for Vascular Biology, Farmington, CT 06030-3501, United States.
Clifford E BerkmanWashington State University, Department of Chemistry, Pullman, WA 99164-4630., United States. Electronic address: cberkman@wsu.edu.

Funding

Near-IR Ratiometric Fluorescence Probes for Assessing Cargo Delivery to Prostate TumorsR21CA256382 · NCI · WASHINGTON STATE UNIVERSITY · PI BERKMAN, CLIFFORD, CAROMILE, LESLIE ANN · 2021 to 2022
$398k
Tumor-Activated Dual Payload-Drug ConjugatesR21CA292338 · NCI · WASHINGTON STATE UNIVERSITY · PI BERKMAN, CLIFFORD · 2024 to 2024
$393k
NCI NIH HHS R21 CA256382NCI NIH HHS R21 CA292338
6 · The paper itself

Abstract

Targeted payload release in cancer cells can be modulated by tuning both the linker, spacer, and the payload chemistries. In previous studies, a PSMA-targeted probe incorporating a 7-amino-4-methylcoumarin (AMC) payload and a PEG linker resulted in predominant payload release in the lysosome (pH ∼5.0). Here, we introduce a second-generation PSMA-targeted turn-on probe with a shorter, hydrophobic linker and a 7-hydroxy-4-methylcoumarin (HMC) payload. Based on pH-dependent kinetic studies, the HMC payload exhibits faster cleavage at a slightly higher pH (pH 5.5), suggesting an earlier release-potentially more in early endosomes than lysosomes. Our results demonstrate that subtle changes in linker and payload structures can alter intracellular release kinetics, offering improved control over the cellular release site, which is critical for optimizing targeted therapeutic and imaging strategies in prostate cancer cells.

Indexed as

Antigens, SurfaceCoumarinsFluorescent DyesGlutamate Carboxypeptidase IIProstatic NeoplasmsCell Line, TumorHumansHydrogen-Ion ConcentrationMaleMolecular StructureOptical ImagingAntigens, SurfaceCoumarinsFluorescent DyesFOLH1 protein, humanGlutamate Carboxypeptidase IIphosphoramidatepH-responsive cleavable linkerPSMAturn-on probe

Identifiers

PMID41482132
PMCPMC13322305

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.