Evidence map›Paper›PMID 41481724›Full record

ArticleScience advances2026

Differentiation between regulated and disrupted growth arrests allows tailoring of effective treatments for antibiotic persistence.

Adi Rotem, Yoav Kaplan, Orit Gefen, Irine Ronin, Alon Gutfreund, Hagai Rappeport, Raya Faigenbaum-Romm, Nitsan Naor, Elisheva Stav, Oded Agam and 1 more

Abstract read
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In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Adi RotemRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0009-0007-0408-6808
Yoav KaplanRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Orit GefenRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0001-7638-4731
Irine RoninRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0009-0002-7623-2089
Alon GutfreundRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0002-3891-2908
Hagai RappeportRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0002-4284-0150
Raya Faigenbaum-RommRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0002-3980-1978
Nitsan NaorRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Elisheva StavRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0009-0006-0447-2509
Oded AgamRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0002-4118-8586
Nathalie Q BalabanRacah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.ORCID 0000-0001-8018-0766

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibiotic persistence, typically attributed to dormant bacteria, is known to be a major cause of treatment failure. However, despite many years of intense research, no clear consensus on its mechanism has emerged. Here, we demonstrate that high survival under antibiotics may originate from two fundamentally different growth-arrest archetypes: either from a regulated growth arrest, leading to a protected dormant cellular state, or from a dysregulated disrupted growth arrest. Using modeling and experimental approaches including transcriptomics, microcalorimetry, and microfluidics, we unveil the characteristics and vulnerabilities of each growth-arrest archetype. In particular, disrupted bacteria show a general impairment of membrane homeostasis. This understanding resolves previous conflicting results regarding characteristics of persisters and allows tailoring treatments that target the different growth-arrested bacteria. The fundamental distinction between regulated and disrupted growth arrests should be broadly relevant for the description of cells under stress.

Indexed as

Anti-Bacterial AgentsBacteriaDrug Resistance, BacterialEscherichia coliGene Expression Regulation, BacterialMicrobial Sensitivity TestsAnti-Bacterial Agents

Identifiers

PMID41481724
PMCPMC12758538

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.