Evidence map›Paper›PMID 41481706›Full record

ArticleScience advances2026

4Pi stimulated Raman scattering for label-free super-resolution chemical imaging.

Jonathan I Kim, Zachary Ellsworth, Erin L Dunnington, Brian S Wong, Nidhi R Mehta, Chisa Zensho, Dan Fu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Jonathan I KimDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0005-3039-2441
Zachary EllsworthDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.
Erin L DunningtonDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0005-7063-8346
Brian S WongDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0001-6405-468X
Nidhi R MehtaDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0006-8273-9125
Chisa ZenshoDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-8494-7685
Dan FuDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0001-9243-8306

Funding

Non-perturbative imaging of intracellular drug exposure and drug response of kinase inhibitors - Admin SuppR35GM133435 · NIGMS · UNIVERSITY OF WASHINGTON · PI Dan Fu · 2019 to 2026
$2.8M
NIGMS NIH HHS R35 GM133435
6 · The paper itself

Abstract

Super-resolution fluorescence microscopy has transformed biological imaging beyond the diffraction limit. However, many biomolecules, nanostructures, drug molecules, and metabolites cannot be easily tagged, requiring a label-free imaging approach. Stimulated Raman scattering (SRS) microscopy is a powerful platform for super-resolution label-free imaging, yet current super-resolution SRS approaches rely on photoswitching, saturation, or sample expansion, which are limited by labeling, photodamage, or signal dilution, respectively. Here, we combine SRS with 4Pi interferometry to enhance axial resolution nearly sevenfold. We report on improvements in imaging sensitivity and axial resolution using 80-nanometer polystyrene beads. Harnessing the improved axial resolution, we demonstrate super-resolution 4Pi-SRS imaging in resolving small lipid droplet structures in mammalian cells and lipid membranes in

Indexed as

Spectrum Analysis, RamanAnimalsEscherichia coliHumansInterferometryLipid DropletsMicroscopy, Fluorescence

Identifiers

PMID41481706
PMCPMC12758514

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.