Evidence map›Paper›PMID 41480145›Full record

ArticlebioRxiv : the preprint server for biology2025

Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy.

Junyu Liu, Talon Chandler, Yue Li, Atharva Agashe, Mingzhe Wei, Yijun Su, Yicong Wu, Tobias I Baskin, Valentin Jaumouillé, Jiji Chen and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Junyu LiuState Key Laboratory of Extreme Optics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Talon ChandlerChan Zuckerberg Biohub, San Francisco, CA, USA.
Yue LiState Key Laboratory for Vegetation Structure, Function and Construction (VegLab), College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Atharva AgasheDepartment of Mechanical Engineering, Virginia Tech, Blacksburg, VA, USA.
Mingzhe WeiState Key Laboratory of Extreme Optics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Yijun SuAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Yicong WuAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Tobias I BaskinDepartment of Biology, University of Massachusetts, Amherst, MA, USA.
Valentin JaumouilléDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Jiji ChenAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Pengcheng XuState Key Laboratory of Extreme Optics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Huihui YeSchool of Communication Engineering, Hangzhou Dianzi University, Hangzhou, China.
Wentao ZhuKey Laboratory for Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China.
Robert S FischerCell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Vinay SwaminathanDepartment of Clinical Sciences, Lund University, Lund, Scania SE-221 00, Sweden.
Amrinder S NainDepartment of Mechanical Engineering, Virginia Tech, Blacksburg, VA, USA.
Shalin B MehtaChan Zuckerberg Biohub, San Francisco, CA, USA.
Patrick J La RiviereWhitman Center, Marine Biological Laboratory, Woods Hole, MA, USA.
Hari ShroffJanelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
Huafeng LiuKey Laboratory for Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China.
Min GuoState Key Laboratory of Extreme Optics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding molecular orientation and density distributions is essential for exploring biological structure and function. Polarized fluorescence microscopy (PFM) provides insights into molecular architecture but struggles to resolve three-dimensional (3D) molecular orientation distributions, particularly in densely labeled or structurally complex specimens. To address this, we introduce the efficient generalized Richardson-Lucy (eGRL) algorithm, a robust framework for reconstructing 3D molecular density and orientation (spatio-angular) distributions from PFM data. By modeling the imaging process in spatio-angular hyperspace, we propose a maximum-likelihood solution enhanced by dimensionality reduction and angular domain transformation to overcome computational challenges. eGRL improves accuracy and efficiency across different PFM implementations, enabling use on standard platforms. We utilize our methods to resolve biological spatio-angular structures and dynamics otherwise impossible to resolve, including the tangential alignment of actin filaments in U2OS cells, nanowire-guided cytoskeletal organization in NIH3T3 cells, rotational actin patterns in live HeLa protrusions, and membrane tension-induced anisotropy in live macrophages.

Identifiers

PMID41480145
PMCPMC12754719

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.