A Head and Neck Cancer Spheroid Model for Phenotypic High-Content Screening of Chemoresistance.

Madita Wandrey, Aya Khamis Hassan, Roland H Stauber, Désirée Gül

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Madita WandreyDepartment of Otorhinolaryngology, Head and Neck Surgery, Molecular and Cellular Oncology, University Medical Center, Mainz, Germany.

Aya Khamis HassanMaxillofacial and Oral Surgery, University Medical Center, Mainz, Germany.

Roland H StauberDepartment of Otorhinolaryngology, Head and Neck Surgery, Molecular and Cellular Oncology, University Medical Center, Mainz, Germany.

Désirée GülDepartment of Otorhinolaryngology, Head and Neck Surgery, Molecular and Cellular Oncology, University Medical Center, Mainz, Germany. guel@uni-mainz.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Resistance against common treatment regimens like chemotherapy is a major concern in current cancer research and clinical disease management. Moreover, in vitro cell culture models cannot always adequately represent the tumor situation in vivo, hampering the analysis of the underlying mechanisms. Efforts have been made to establish three-dimensional (3D) cancer cell models, such as spheroids or organoids, to mimick the in vivo situation of the tumor more closely. Here, we describe the establishment of a 3D spheroid model of head and neck squamous cell carcinoma (HNSCC), allowing for the study of chemoresistance. The analysis of relevant phenotypic readouts using a high-content screening platform enables the objective evaluation and monitoring of spheroids during chemotherapeutic treatment. This model also represents a starting point for the evaluation of a precision medicine-based management of HNSCC patients.

Indexed as

Antineoplastic AgentsCell Culture TechniquesDrug Resistance, NeoplasmHead and Neck NeoplasmsHigh-Throughput Screening AssaysSpheroids, CellularSquamous Cell Carcinoma of Head and NeckCell Line, TumorDrug Screening Assays, AntitumorHumansPhenotypeAntineoplastic AgentsChemoresistanceHigh-content screeningHNSCCPhenotypic screeningPrecision medicineSpheroid cell culture

Identifiers

PMID41479057

DOI10.3390/ijms24054675

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.