Spinal Spheroids as Screening Platform for Peripheral Neuropathies.

Elizabeth H Jacobs, Jessica Medina, Christopher Yanick, Renata Maciel, Mario Saporta

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Elizabeth H JacobsDepartment of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA.

Jessica MedinaDepartment of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA.

Christopher YanickDepartment of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA.

Renata MacielDepartment of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA.

Mario SaportaDepartment of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA. mas638@med.miami.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The lack of effective disease-modifying therapies for axonopathies highlights the need for novel preclinical models suitable for treatment development. Two-dimensional neuronal cultures lack the directional axonal distribution required to investigate length-dependent processes such as peripheral neuropathies. To optimize this well-established model system, we developed a robust human platform to study axonal morphology and physiology based on three-dimensional motor neuron cultures (i.e., spinal spheroids). We differentiate motor neurons from human induced pluripotent stem cells, purify them by magnetic sorting, and culture them in suspension until they form spheroids. Axons are allowed to grow out of plated spinal spheroids in a radial fashion at an average rate of 200 micrometers/day and reach up to 1 cm in length. This system is optimized for morphological analysis, including high content imaging, investigation of axonal protein expression, and time-lapse imaging of axonal transport.

Indexed as

Peripheral Nervous System DiseasesSpheroids, CellularSpinal CordAxonal TransportAxonsCell Culture TechniquesCell DifferentiationCells, CulturedHumansInduced Pluripotent Stem CellsMotor NeuronsTime-Lapse ImagingCharcot-Marie-Tooth diseaseHigh content imagingInduced pluripotent stem cellsMotor neuronsNeuropathySpinal spheroids

Identifiers

PMID41479056

DOI10.1002/cpt.1718

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.