Semi-automated Detection of Late Cytokinetic Bridges.

Mikaël Boullé, Fabrice Agou

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mikaël BoulléCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, Paris, France. mikael.boulle@uclouvain.be.

Fabrice AgouCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, Paris, France. fabrice.agou@pasteur.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During cytoplasmic division, the intercellular bridge is formed with a central midbody. In our opinion, more tools are required for investigation of the late molecular events leading to cytokinetic abscission. In this chapter, we briefly review the many available methods to screen for cytokinetic defects and present a protocol for image-based detection of late cytokinetic bridges. A simple immunofluorescence staining of the fixed cytoplasms, nuclei, and the midbodies, analyzed with tailored software, can reproducibly estimate the frequency of intercellular bridges. Here, we describe data collection and analysis in single cells upon inhibition by RNA interference using fluorescence microscopy.

Indexed as

CytokinesisCell NucleusCytoplasmFluorescent Antibody TechniqueHumansImage Processing, Computer-AssistedMicroscopy, FluorescenceRNA InterferenceSoftwareCytokinesisEukaryotic cellsHigh-content imagingIntercellular bridgeMidbody

Identifiers

PMID41479054

DOI10.1007/978-3-030-44826-4_25

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.