ArticleMethods in molecular biology (Clifton, N.J.)2026
Cell Painting Protocol to Characterize Morphological Profiles of Large Compound Collections Using the EU-OPENSCREEN Library.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Morphological profiling with the Cell Painting assay started a new era in phenotypic screening approaches by harnessing comprehensive morphological profiles of cellular perturbations. This enables an unbiased characterization of the effects of small chemical compounds. We established and extensively validated a Cell Painting protocol to screen the compound libraries of the European initiative EU-OPENSCREEN ( www.eu-openscreen.eu ) in order to find robust and reproducible links between the known target or the pathway mechanism of each compound to the phenotypic profile. In this chapter, we describe the Cell Painting procedure, image analysis, and the downstream data processing procedures.
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