T Cell Assay to Track Presentation of an Influenza Hemagglutinin Epitope Presented by the MHC-II Protein HLA-DR1.

Karthik M Ramesh, Liying Lu, Lawrence J Stern

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Karthik M RameshDepartment of Pathology, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Liying LuDepartment of Pathology, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Lawrence J SternDepartment of Pathology, University of Massachusetts Chan Medical School, Worcester, MA, USA. lawrence.stern@umassmed.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antigen presentation is a specialized function of the immune system where peptides generated from cellular or internalized proteins are presented on Major Histocompatibility Complex molecules on the surface of antigen-presenting cells for recognition by T cells. The binding of these MHC-peptide complexes to hypervariable receptors on the surface of a T cell leads to its activation. T cell activation induces increases in cell-surface activation markers such as CD69, which can be tracked by flow cytometry. Here we describe a method that uses flow cytometry and a recombinant T cell to track the presentation of an MHC-II HLA-DR1 restricted epitope generated from the influenza hemagglutinin protein.

Indexed as

Antigen PresentationEpitopesEpitopes, T-LymphocyteHemagglutinin Glycoproteins, Influenza VirusHLA-DR1 AntigenT-LymphocytesFlow CytometryHumansLymphocyte ActivationEpitopesEpitopes, T-LymphocyteHemagglutinin Glycoproteins, Influenza VirusHLA-DR1 AntigenAntigen presentationAntigen-presenting cellB cellFlow cytometryHemagglutininHLA-DRInfluenzaMajor histocompatibility complexPeptideT cell

Identifiers

PMID41479015

DOI10.1016/S1074-7613(00)80169-7

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.