ArticleMethods in molecular biology (Clifton, N.J.)2026
Expression and Functional Characterization of Recombinant HLA-DM.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
HLA-DM is a nonclassical MHC class II molecule that plays an essential role in antigen presentation by catalyzing peptide exchange on MHC class II molecules, ensuring the presentation of stable peptide-MHC complexes to CD4+ T cells. This chapter describes detailed protocols for the production and functional analysis of recombinant HLA-DM and HLA-DR proteins. Using a baculovirus expression system in Spodoptera frugiperda (Sf9) cells, we outline step-by-step methods for bacmid preparation, baculovirus amplification, and large-scale protein expression. Comprehensive purification strategies, including affinity chromatography and size exclusion chromatography, are provided to ensure high-purity protein yields. Additionally, we describe functional assays, utilizing fluorescence polarization-based peptide exchange to evaluate HLA-DM activity. These protocols enable the reproducible production of recombinant HLA-DM and HLA-DR, providing a reliable foundation for researchers conducting functional and mechanistic studies on catalyzed peptide exchange.
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