Evidence map›Paper›PMID 41478987›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Optimized Whole-Mount X-gal Staining to Detect Hedgehog Signaling Activity in Basal Cell Carcinoma: A Platform for Spatial Integration with Immune Analysis.

Hao Wang, Yucui Zhu, Arianna L Kim

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hao WangDepartment of Dermatology, Columbia University Irving Medical Center, New York, NY, USA.
Yucui ZhuDepartment of Dermatology, Columbia University Irving Medical Center, New York, NY, USA.
Arianna L KimDepartment of Dermatology, Columbia University Irving Medical Center, New York, NY, USA. ak309@cumc.columbia.edu.

Funding

Molecular Mechanisms Underlying the Prevention of BCC ResistanceR01ES030481 · NIEHS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BICKERS, DAVID RINSEY, KIM, ARIANNA L · 2020 to 2024
$1.8M
Targeting Tumor-Driven Immune Tolerance to Overcome Resistance to Hedgehog InhibitionR21CA301201 · NCI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI ARIANNA L KIM · 2025 to 2026
$423k
NCI NIH HHS R21 CA301201NIEHS NIH HHS R01 ES030481
6 · The paper itself

Abstract

Whole-mount X-gal staining is a classical histochemical method for detecting β-galactosidase (LacZ) expression in fixed tissues, providing spatial resolution of gene activity in situ. In cancer research, LacZ serves as a versatile reporter for monitoring gene activation, tracing cell lineage in genetically engineered models, and assessing cellular responses to oncogenic signaling within the tumor microenvironment. Here, we present an optimized protocol for the in situ visualization of LacZ

Indexed as

Basal Cell CarcinomaHedgehog ProteinsSignal TransductionSkin NeoplasmsStaining and LabelingAnimalsbeta-GalactosidaseDisease Models, AnimalGenes, ReporterHumansMiceTumor Microenvironmentbeta-GalactosidaseHedgehog ProteinsBasal cell carcinomaHedgehog pathwayImmune microenvironmentLacZ reporter

Identifiers

PMID41478987
PMCPMC12994386

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.