ArticleMethods in molecular biology (Clifton, N.J.)2026
Proximity Ligation Assay to Detect Protein-Protein Interaction in Cancer Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Combining immunoassays with nucleic acid-based amplification and detection, the proximity ligation assay (PLA) is a useful tool for immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein-protein interactions in fixed cells and tissue samples. Here, we present a detailed procedure for detecting protein-protein interactions in cancer cells with a commercially available Naveni® in situ proximity ligation technology to assist the researcher in successfully performing the experiments.
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