ArticleMethods in molecular biology (Clifton, N.J.)2026
Masson's Trichrome Staining Technique to Evaluate Tissue Fibrosis.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle (Animals : an open access journal from MDPI · 2026Article
- Evaluation of Curcumin as a Supplement to Improve Mitotane Effects on Adrenocortical Carcinoma.Cells · 2026Article
- Use of Artificial Intelligence Model Associated with Masson's Trichrome Staining as a Predictor of Muscle Invasion in Bladder Cancer.International journal of molecular sciences · 2026Article
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Authors and funding
2 authors.
Funding
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Abstract
Masson's Trichrome staining is the most robust technique to evaluate tissue fibrosis. As the name indicates, trichrome staining utilizes three dyes that stain nuclei, cytoplasm, and connective tissue fibers and help visualize fibrosis to identify the disease state. Here, we describe a detailed Masson's trichrome staining technique utilizing two types of liver tissues harvested from C57BL/6 mice. One group of mice was fed normal chow, while the other group was fed a high-fat-fructose diet for 22 weeks and developed fibrosis. The protocol consists of tissue processing, embedding, sectioning, and staining. After the tissue sectioning is completed, it takes approximately 4 h to finish the trichrome staining.
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