Evidence map›Paper›PMID 41478965›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Evaluating Nuclear Levels of PD-L1 in Ovarian Cancer Cells by Western Blotting.

Suprataptha U Reddy, Fatema Zohra Sadia, Tanjia Mobin, Brenden N Jung, Ivana Vancurova

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Suprataptha U ReddyDepartment of Biological Sciences, St. John's University, Queens, NY, USA.
Fatema Zohra SadiaDepartment of Biological Sciences, St. John's University, Queens, NY, USA.
Tanjia MobinDepartment of Biological Sciences, St. John's University, Queens, NY, USA.
Brenden N JungDepartment of Biological Sciences, St. John's University, Queens, NY, USA.
Ivana VancurovaDepartment of Biological Sciences, St. John's University, Queens, NY, USA. vancuroi@stjohns.edu.

Funding

Regulation of mRNA homeostasis by PD-L1R16GM149263 · NIGMS · ST. JOHN'S UNIVERSITY · PI Ivana Vancurova · 2023 to 2026
$656k
NIGMS NIH HHS R16 GM149263
6 · The paper itself

Abstract

Nuclear accumulation of immune checkpoint PD-L1 has been associated with increased chemoresistance and cancer progression. Since nuclear PD-L1 is largely inaccessible to immunotherapies blocking cell surface PD-L1 signaling and to analytical methods measuring surface PD-L1 expression on cancer cells, it is important to develop convenient and reliable methods to measure the nuclear PD-L1 levels. Here, we describe a simple and efficient protocol that uses biochemical preparation of cytoplasmic and nuclear extracts, which are then analyzed by western blotting using PD-L1 monoclonal antibody that specifically recognizes endogenous PD-L1.

Indexed as

B7-H1 AntigenBlotting, WesternCell NucleusOvarian NeoplasmsCell Line, TumorFemaleHumansB7-H1 AntigenCD274 protein, humanCancer immunotherapyInterferon-γNuclear accumulationNuclear PD-L1Ovarian cancerPD-L1Western blotting

Identifiers

PMID41478965

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.