Flow Cytometry Analysis of IFNγ-Induced PD-L1 Cell Surface Expression in Ovarian Cancer Cells.

Sveta Padmanabhan, Yue Zou, Ivana Vancurova

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sveta PadmanabhanDepartment of Biology, St. John's University, Queens, NY, USA.

Yue ZouDepartment of Biology, St. John's University, Queens, NY, USA.

Ivana VancurovaDepartment of Biology, St. John's University, Queens, NY, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunotherapies blocking immune checkpoint PD-1/PD-L1 signaling have shown great promise in a variety of cancers, but most patients fail to respond or develop resistance. One of the factors determining the responsiveness to PD-1/PD-L1 targeting immunotherapies is surface expression of PD-L1 on cancer cells. Here we describe a flow cytometry protocol for the analysis of cell surface expression of PD-L1 induced by IFNγ in ovarian cancer cells, by using antibody that specifically recognizes the extracellular domain of PD-L1.

Indexed as

B7-H1 AntigenFlow CytometryInterferon-gammaOvarian NeoplasmsCell Line, TumorFemaleGene Expression Regulation, NeoplasticHumansB7-H1 AntigenCD274 protein, humanInterferon-gammaCancer immunotherapyFlow cytometryImmune checkpointsInterferon-γOvarian cancerPD-L1

Identifiers

PMID41478964

DOI10.1007/978-1-0716-0247-8_19

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.