Evidence map›Paper›PMID 41478956›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Whole Genome Amplification of Microbial DNA from Host-Depleted Clinical Samples.

Carl Halford, Lluis Moragues-Solanas, Simon A Weller, Matthew Gilmour

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Carl HalfordDefence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK. chalford@dstl.gov.uk.
Lluis Moragues-SolanasMicrobiology, Biology Department, Universitat de les Illes Balears, Palma, Balearic Islands, Spain.
Simon A WellerDefence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK.
Matthew GilmourNorwich Medical School, University of East Anglia, Norwich, Norfolk, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clinical metagenomics (CMg) involves the untargeted sequencing of the genetic content of samples collected from patients and is a highly promising method for the diagnosis of infectious disease. Depending on the sample type, CMg can be reliant on the removal of the host genetic material from the sample to support detection of microbial pathogens, and this selective process (or an otherwise low abundance of microbial cells in the sample) may result in concentrations of DNA too low for productive sequencing. Whole genome amplification (WGA), the nonselective amplification of the total DNA of a sample, can be applied to significantly increase the concentration of DNA and enable CMg sequencing. This chapter describes the methods for the amplification of microbial DNA extracted from host-depleted wound swab samples using the GenomiPhi

Indexed as

DNA, BacterialMetagenomicsNucleic Acid Amplification TechniquesHigh-Throughput Nucleotide SequencingHumansSequence Analysis, DNADNA, BacterialClinical metagenomicsDe-branchingHost-depletionWhole genome amplification

Identifiers

PMID41478956

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.