Laser Microdissection and Near Single-Cell Whole Genome Amplification.

Roberto Cruz-Flores, Jorge Cáceres-Martínez, Arun K Dhar

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Roberto Cruz-FloresAquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, AZ, USA. adhar@arizona.edu.

Jorge Cáceres-MartínezCentro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California, Mexico.

Arun K DharCentro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California, Mexico.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Laser microdissection (LMD) and whole genome amplification (WGA) are powerful techniques that integrate molecular and histological approaches to enable the precise selection of a minimal number of virus-infected cells-down to near single-cell resolution-and the subsequent generation of whole viral genomes with minimal host DNA interference. This chapter presents a detailed protocol for LMD and near single-cell WGA, specifically optimized for the recovery and sequencing of viral genomes from formalin-fixed paraffin-embedded (FFPE) tissues. The method allows for the targeted isolation of infected cells, thereby reducing host genomic background and enhancing the detection of pathogen-specific signals for downstream next-generation sequencing (NGS). The protocol includes steps for tissue section preparation, cell isolation via LMD, DNA extraction using the PicoPure DNA Extraction Kit, and unbiased genome amplification using the SeqPlex DNA Amplification Kit-ensuring high-quality nucleic acid recovery suitable for NGS workflows.

Indexed as

Genome, ViralLaser Capture MicrodissectionNucleic Acid Amplification TechniquesSingle-Cell AnalysisDNA, ViralGenomicsHigh-Throughput Nucleotide SequencingHumansParaffin EmbeddingTissue FixationDNA, ViralFFPELaser microdissectionMetagenomicsPathogen detectionSeqPlexShrimp virusesSingle-cell genomicsWhole genome amplification

Identifiers

PMID41478955

DOI10.1016/j.mimet.2022.106476

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.