Evidence map›Paper›PMID 41478948›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Whole-Genome Amplification of Single Circulating Tumor Cells from Mice Xenografts.

Nuria Estévez-Gómez, Cristóbal Fernández-Santiago, Roberto Piñeiro, David Posada

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nuria Estévez-GómezCINBIO, Universidade de Vigo, Vigo, Spain. nuestevez@uvigo.es.
Cristóbal Fernández-SantiagoOncomet, Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Roberto PiñeiroOncomet, Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
David PosadaCINBIO, Universidade de Vigo, Vigo, Spain. dposada@uvigo.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genomic analysis of circulating tumor cells (CTCs) can help us identify their specific characteristics and reveal intratumor heterogeneity while also reducing the need for invasive sampling. However, studying single CTCs can be challenging, as whole-genome amplification (WGA) is needed to obtain enough genetic material for high-throughput sequencing. Here, we present a protocol for isolating single CTCs from mouse xenografts, followed by WGA using the Ampli1WGA Plus kit.

Indexed as

NeoplasmsNeoplastic Cells, CirculatingNucleic Acid Amplification TechniquesSingle-Cell AnalysisAnimalsCell Line, TumorGenomicsHeterograftsHigh-Throughput Nucleotide SequencingHumansMiceAmpli1Cancer genomicsCirculating tumor cellsLiquid biopsySingle-cell genomicsSingle-cell whole-genome amplificationXenografts

Identifiers

PMID41478948

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.