ArticleMethods in molecular biology (Clifton, N.J.)2026
A Tailing Method of Quality Control on MALBAC-Based Whole-Genome Amplification Products.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Single-cell whole-genome sequencing (scWGS) has illuminated new perspectives on genetic diversity across different cellular conditions. Despite this, the amplification of DNA from sparsely populated formalin-fixed tissues during the whole-genome amplification (WGA) process remains a formidable challenge. We introduce an enhanced quality control protocol for WGA products generated using the multiple annealing and looping-based amplification cycles (MALBAC) technique. This approach presents a cost-efficient, high-throughput quality control method, delivering a precise assessment of WGA efficacy with 100% accuracy on a test set of 128 samples.
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