Evidence map›Paper›PMID 41478944›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Whole-Genome Amplification for Circulating Tumor Cells.

Masato Kojima, Eiso Hiyama

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Masato KojimaDepartment of Pediatric Surgery, Hiroshima University Hospital, Hiroshima, Japan. mk1019@hiroshima-u.ac.jp.
Eiso HiyamaDepartment of Biomedical Science, Natural Science Center for Basic Research and Development (N-BARD), Hiroshima University, Hiroshima, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circulating tumor cells (CTCs) are cancer cells that dissociate from primary and metastatic sites. The "liquid biopsy" analysis of CTCs is becoming attractive due to the easy access to blood. CTCs are thought to be the source of tumor progression and metastasis; genetic analysis of single CTCs allows the identification and characterization of tumor heterogeneity and malignancy. In clinical settings, the analysis of single CTCs can provide insights into tumor evolution and lead to precision cancer therapy. Genetic analysis of single cells is restricted by the low amounts of DNA (6-7 pg); therefore, whole-genome amplification (WGA) is required for downstream genetic analysis. In this chapter, we describe in detail the protocols from isolation of CTC from blood to WGA and quality check for downstream genetic analysis.

Indexed as

Genome, HumanNeoplasmsNeoplastic Cells, CirculatingNucleic Acid Amplification TechniquesBiomarkers, TumorCell SeparationHumansLiquid BiopsySingle-Cell AnalysisBiomarkers, TumorCirculating tumor cellLiquid biopsyPrecision medicineSingle-cell analysisWhole-genome amplification

Identifiers

PMID41478944

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.