Evidence map›Paper›PMID 41478933›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Intravital Microscopy of Mesenteric and Brain Vessels: A Valuable Tool for Leukocyte Recruitment Evaluation.

William Antonio Gonçalves, Lara Penna, Isadora Oliveira Gondim, Vinicius Leal Pitcella, Sabrina Berger da Silva, Luan Lopes Menezes, Vanessa Pinho, Mauro Martins Teixeira, Barbara Maximino Rezende

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

William Antonio GonçalvesDepartamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0001-6960-4337
Lara PennaDepartamento de Patologia Geral, Programa de Pós-Graduação em Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0001-5234-6888
Isadora Oliveira GondimDepartamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0001-7709-6336
Vinicius Leal PitcellaDepartamento de Patologia Geral, Programa de Pós-Graduação em Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0003-3416-0742
Sabrina Berger da SilvaDepartamento de Patologia Geral, Programa de Pós-Graduação em Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0009-0004-7550-4307
Luan Lopes MenezesDepartamento de Patologia Geral, Programa de Pós-Graduação em Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0009-0001-3914-0487
Vanessa PinhoDepartamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0003-1038-2324
Mauro Martins TeixeiraDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0002-6944-3008
Barbara Maximino RezendeDepartamento de Patologia Geral, Programa de Pós-Graduação em Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID https://orcid.org/0000-0001-7959-7732

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Graft-versus-host disease (GVHD) is the most serious complication limiting the clinical utility of allogeneic hematopoietic stem cell transplantation (HSCT), in which lymphocytes of donors (graft) are activated in response to the host antigen. This disease is related to increased inflammatory response through releasing inflammatory mediators and recruiting defense cells, such as leukocytes. Intravital microscopy is a technique that allows the observation of leukocyte interactions in vivo. It can be performed using conventional light microscopy in thin and transparent tissues or epifluorescence microscopy in solid and non-transparent tissues. Here, we describe the procedure to execute this method at the mesenteric and brain vessels of mice subjected to GVHD.

Indexed as

BrainGraft vs Host DiseaseIntravital MicroscopyLeukocytesMesenteryAnimalsHematopoietic Stem Cell TransplantationMiceBrain vesselsFluorescent labelingInflammationIntravital microscopy; graft-versus-host-diseaseLeukocyte recruitmentMesenteric vessels

Identifiers

PMID41478933

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.