Combining UV-Vis Absorption and FRET Melting Curves to Screen the Thermodynamic Stability and Folding Pathways of Nucleic Acids In Vitro.

Vanessa Schumann, Paul Lehmann, Josephine Meitzner, Lynn V Reuss, Richard Börner

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Vanessa SchumannLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.

Paul LehmannLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.

Josephine MeitznerLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.

Lynn V ReussLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.

Richard BörnerLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany. richard.boerner@hs-mittweida.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a high-throughput method for characterizing nucleic acid folding pathways and thermodynamic stability by combining UV-vis absorption and ensemble FRET melting curve analyses. While UV-vis provides global information on NA stability by monitoring base stacking, FRET probes local conformational transitions via fluorophore distance changes. This dual approach allows us to resolve both global and site-specific folding trajectories. Automated data acquisition and analysis are implemented via Python-based Jupyter notebooks. Using model DNA and RNA constructs, we demonstrate ion-dependent, temperature-induced unfolding behavior and highlight the complementarity of the two techniques in probing NA folding landscapes.

Indexed as

DNAFluorescence Resonance Energy TransferNucleic Acid ConformationNucleic AcidsRNANucleic Acid DenaturationSpectrophotometry, UltravioletThermodynamicsDNANucleic AcidsRNAFRETHigh throughputNucleic acid foldingThermal meltingThermodynamic stabilityUV-vis absorption

Identifiers

PMID41478905

DOI10.1007/978-1-60327-429-6_1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.