Evidence map›Paper›PMID 41478904›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Application of Ultraviolet (UV) and Circular Dichroism (CD) Spectroscopies to Study RNA-RNA Interactions of Regulatory RNAs of Bacteriophage Origin.

Natalia Lewandowska, Sylwia Bloch, Agnieszka Chylewska, Aleksandra M Dąbrowska, Monika Mazur, Joanna Zwolenkiewicz, Wojciech Wesołowski, Aleksandra Łukasiak, Emilia Węglińska, Mikołaj Olejniczak and 2 more

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Natalia LewandowskaDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0002-6652-9711
Sylwia BlochDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0002-3451-3619
Agnieszka ChylewskaDepartment of Bioinorganic Chemistry, Faculty of Chemistry, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0001-7413-1503
Aleksandra M DąbrowskaDepartment of Bioinorganic Chemistry, Faculty of Chemistry, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0002-1953-6636
Monika MazurInstitute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.ORCID https://orcid.org/0000-0003-2525-4755
Joanna ZwolenkiewiczInstitute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.ORCID https://orcid.org/0000-0003-3024-0702
Wojciech WesołowskiDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0003-4631-0648
Aleksandra ŁukasiakDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0002-5202-4261
Emilia WęglińskaDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0009-0000-8097-4160
Mikołaj OlejniczakInstitute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.ORCID https://orcid.org/0000-0001-5949-3944
Grzegorz WęgrzynDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland.ORCID https://orcid.org/0000-0003-4042-7466
Bożena Nejman-FaleńczykDepartment of Molecular Biology, Laboratory of Biology and Biotechnology of Bacteriophages, University of Gdańsk, Gdansk, Poland. bozena.nejman-falenczyk@ug.edu.pl.ORCID https://orcid.org/0000-0003-4468-0733

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this chapter, we present the possibility of using ultraviolet (UV) and circular dichroism (CD) spectroscopies to analyze RNA-RNA interactions as an alternative or complementary technique to the classical RNA gel mobility shift assay. We present relevant, detailed protocols and the results obtained for a specific biological model to investigate all three methods. In this model, we studied the binding of small noncoding sRNA molecules of bacteriophage origin to fragments of bacterial mRNAs that were selected based on in silico analysis. We conclude that spectroscopic methods can be especially useful in analyzing the interactions of small RNAs with target transcripts and in the experimental validation of their target sites of action.

Indexed as

BacteriophagesCircular DichroismRNA, Small UntranslatedRNA, ViralNucleic Acid ConformationRNA, MessengerSpectrophotometry, UltravioletUltraviolet RaysRNA, MessengerRNA, Small UntranslatedRNA, ViralCircular dichroism (CD) spectroscopyGene expressionRegulatory noncoding sRNAsRNARNA gel mobility shift assayRNA interactionUltraviolet (UV) spectroscopy

Identifiers

PMID41478904

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.