ArticleMethods in molecular biology (Clifton, N.J.)2026
Analysis of Protein-RNA Interactions by Protein-Induced Fluorescence Enhancement (PIFE).
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In this chapter, we provide an overview of a fluorescence technique, known as "Protein-Induced Fluorescence Enhancement" (PIFE), to follow the association between a protein and an RNA. Unlike Förster-type resonance energy transfer (FRET), which requires at least two dyes, PIFE utilizes a single fluorophore attached to the RNA, with an unlabeled protein interacting with the nucleic acid. We discuss how both ensemble and single-molecule PIFE studies can be employed to explore molecular interactions and to measure the strength of the interactions in solution with low sample consumption.
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