Evidence map›Paper›PMID 41478514›Full record

ArticleInsect biochemistry and molecular biology2026

Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis in Drosophila melanogaster.

Daiki Fujinaga, Yuya Ohhara, Naoki Okamoto, Hannah Chu, Kerry E Mauck, Naoki Yamanaka

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Daiki FujinagaDepartment of Entomology, University of California, Riverside, CA, 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Yuya OhharaSchool of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka, 422-8526, Japan; Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka, 422-8526, Japan.
Naoki OkamotoDepartment of Entomology, University of California, Riverside, CA, 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan.
Hannah ChuDepartment of Entomology, University of California, Riverside, CA, 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Kerry E MauckDepartment of Entomology, University of California, Riverside, CA, 92521, USA.
Naoki YamanakaDepartment of Entomology, University of California, Riverside, CA, 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA. Electronic address: naoki.yamanaka@ucr.edu.

Funding

Juvenile hormone transporters in disease vector physiologyR01AI171032 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Naoki Yamanaka · 2023 to 2026
$1.5M
Molecular genetics of signaling lipidsR35GM153331 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Naoki Yamanaka · 2024 to 2026
$1.2M
NIAID NIH HHS R01 AI171032NIGMS NIH HHS R35 GM153331
6 · The paper itself

Abstract

Juvenile hormones (JHs) mediate various biological processes such as development and reproduction in insects. Although pleiotropic functions of JHs are well investigated in the fruit fly Drosophila melanogaster, their biosynthetic mechanisms are less well understood, partly because many JH biosynthetic enzymes still remain unidentified in this important model species. Here we report that two cytochrome P450 (CYP) epoxidases mediate JH biosynthesis in D. melanogaster. In addition to previously reported Cyp6g2, a second epoxidase, Cyp6a13, also functions in the corpus allatum, the major JH biosynthetic endocrine gland. Combined mutations of the genes encoding these enzymes cause developmental and reproductive defects, which can be rescued by JH application. JH biosynthetic functions of these genes were further confirmed by using a heterologous expression system and ex vivo tissue culture. Collectively, our results indicate that these two CYP epoxidases function cooperatively to mediate JH biosynthesis in D. melanogaster.

Indexed as

Cytochrome P-450 Enzyme SystemDrosophila melanogasterDrosophila ProteinsJuvenile HormonesAnimalsCorpora AllataFemaleCytochrome P-450 Enzyme SystemDrosophila ProteinsJuvenile HormonesCytochrome P450Drosophila melanogasterJuvenile hormoneMass spectrometryMetamorphosis

Identifiers

PMID41478514
PMCPMC13285967

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.