Evidence map›Paper›PMID 41477872›Full record

ArticleScience advances2026

Engineered anchoring aptamers induce relocalization and functional inactivation of transcription factors.

Neng Ling, Hui Wu, Yibin Zhang, Shuang Yao, Fangyu Liu, Xinyue Tang, Tiantian Xie, Yijun Yuan, Xianhua Wei, Jing Dai and 9 more

Abstract read
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In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Neng LingOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID 0000-0001-8274-4414
Hui WuThe Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
Yibin ZhangDepartment of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.ORCID 0000-0002-9332-3559
Shuang YaoOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Fangyu LiuMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Xinyue TangMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Tiantian XieMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Yijun YuanMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Xianhua WeiMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Jing DaiMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Wenjing ChenMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Ming YanMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Zhiyan WangMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Tianhuan PengMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.ORCID 0000-0002-2229-2687
Yanchen ZhangOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Hongmei WangOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Jianzhang WuOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID 0000-0002-2782-745X
Mao YeMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.ORCID 0000-0002-4798-1825
Wencan WuOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID 0000-0003-3019-5702

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proper nucleocytoplasmic distribution of transcription factors (TFs) is crucial for cellular homeostasis. Mislocalization of TFs contributes to cancers, making TF relocalization a promising anticancer therapeutic strategy. We engineered anchoring aptamers as a platform for relocalizing TFs. Anchoring aptamers are heterobifunctional nucleic acids consisting of two ligands joined by a linker: one targeting the plasma membrane-anchored protein and the other recruiting the TF. As a proof of concept, we used the Ra1 aptamer targeting Ras and the DNA ligand specific for p65 or E2F1 to construct anchoring aptamers. Simultaneous binding of the anchoring aptamers to Ras and either p65 or E2F1 effectively induces cytoplasmic relocalization and functional inactivation of p65 or E2F1. Using a lentiviral expression system of anchoring aptamers, we achieved sustained cytoplasmic retention of p65 and marked inhibition of tumor growth. This work establishes a universal platform for TF relocalization, offering promising opportunities for innovative anticancer therapeutic strategies.

Indexed as

Aptamers, NucleotideE2F1 Transcription FactorTranscription FactorsAnimalsCell Line, TumorCytoplasmHumansMiceAptamers, NucleotideE2F1 protein, humanE2F1 Transcription FactorTranscription Factors

Identifiers

PMID41477872
PMCPMC12757074

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.