ArticleThe Yale journal of biology and medicine2025
A Triplex PCR Assay for Simultaneous Detection of Non-viral STDs in Both Dry and Wet Swabs, Offering a Rapid and Cost-Effective Diagnostic Tool.
Article in The Yale journal of biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Sexually transmitted diseases (STDs) remain a major global public health challenge because their health consequences lead to severe complications, especially among sexually active populations. Methods: Designed and evaluated the performance of a multiplex polymerase chain reaction (PCR) in both dry and wet samples to detect three non-viral STDs, which were diagnosed based on syndromic case management (SCM) and uniplex PCR tests. We collected samples in duplicate (n=133), one as dry swab and the other swab was immediately put in transport medium and brought to the laboratory at ambient temperature within 4-5 hours. The total DNA was isolated from dry and wet swabs. Results: Out of 133 samples, six were positive for all three infections in both dry and wet swabs, while 127 were negative for all three. All clinical samples were then tested using uniplex PCR for each pathogen and also using triplex PCR for clinical evaluations. Conclusions: Multiplex PCR developed in-house is an effective test for simultaneous diagnosis of
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