Phenolic Compounds and Flavonoids in Various Extracts of

Background: Flavonoids, which are bioactive compounds found in medicinal plant extracts, are recognized for their significant therapeutic properties. To efficiently isolate these compounds from fresh plant materials, it is essential to optimize extraction methods. The most widely used approach involves solvent extraction using solvents such as methanol, ethanol, acetone, and hexane. This study aimed to determine the optimal extraction methods for isolating phenolic compounds and flavonoids from callus and regenerating plants of two medicinal species of the genus Objectives: The present study represents the first attempt to determine the phenolic compounds in regenerating plants obtained through in vitro methods. Methods: In vitro regenerated plants of Results: We then analyzed methanol, ethanol, and hexane extracts from in vitro Conclusions: This study focused on the declining wild medicinal herbs in natural populations, providing an opportunity for systematic in vitro propagation year-round. This approach aims to establish these herbs as alternative sources for extracting biologically active secondary metabolites such as flavonoids.