ArticleFrontiers in endocrinology2025
Molecular characterization of the murine Leydig cell lines TM3 and MLTC-1.
Article in Frontiers in endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Lycopene Alleviates T-2 Toxin-Induced Testosterone Synthesis Disorder by Inhibiting ROS/p38/TORC2/CREB Axis.Antioxidants (Basel, Switzerland) · 2026Article
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6 authors.
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Abstract
Introduction: Leydig cells (LCs) are located in the interstitium of the testes and are characterized as steroid hormone-producing cells that are crucial for testicular function in maintaining spermatogenesis. Disruptions in their function, for instance due to genetic defects, endocrine disruptors, or age-related changes, often lead to hypogonadism and are among the main causes of male infertility. Therefore, reliable Methods: We used non-tumorigenic TM3 cells and tumorigenic MLTC-1 cells, two commercially available LCs, and subjected them to a comprehensive molecular and genetic comparative analysis. Results and discussion: Light microscopy and F-actin staining by Phalloidin revealed the typical polygonal LC shape of both cell lines growing in clusters with tight cell-cell contacts. Both cell lines contain endogenous lipid droplets (LDs) and synthesis was inducible with oleic acid. Transcriptomic analysis and RT-qPCR verified the expression of different steroidogenesis-associated LD genes including members of the PLIN family and lipolysis enzymes (e.g.
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