ArticleJournal of orthopaedic surgery and research2025
Macrophage-derived exosomal miR-466i-5p and miR-365-2-5p stimulated by BMP-2 promote osteogenesis.
Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Beyond jump distance: modular dual mobility versus single mobility cups in robotic total hip arthroplasty - simulation of hip kinematics and impingement.International orthopaedics · 2026Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundMacrophage-derived exosomes and exosomal microRNAs (miRNAs) are critical mediators of bone marrow mesenchymal stem cell (BMSC) differentiation. However, their regulatory mechanisms under bone morphogenetic protein 2 (BMP2) stimulation remain unclear.
methodsThis study identified macrophage-derived exosomes stimulated or unstimulated by BMP2 (BMP2-Exos and DMEM-Exos, respectively) and co-cultured them with BMSCs to assess the effects of BMP2-Exos on BMSC osteogenic differentiation (n = 3). Bioinformatic methods were used to analyze differentially expressed miRNAs, and RNA sequencing (RNA-Seq) was used to examine the exosomal miRNA expression profiles of BMP2-Exos and DMEM-Exos to elucidate underlying mechanisms.
resultsSix miRNAs were upregulated and one miRNA was downregulated after osteogenic induction. Gene ontology and pathway analyses revealed that the target genes of differentially expressed exosomal miRNAs regulate various biological processes, including protein binding, metabolic processes, and biological regulation, and are enriched in osteogenic differentiation-related pathways, such as the PI3K-Akt signaling pathway and regulation of the actin cytoskeleton. Bioinformatic analysis identified elevated expression of microRNA-466i-5p (miR-466i-5p) and microRNA-365-2-5p (miR-365-2-5p) in BMP2-Exos, which may promote the osteogenic differentiation of BMSCs.
conclusionsBMP2-activated macrophage-derived exosomes regulate BMSC osteogenic differentiation through miR-466i-5p and miR-365-2-5p, thereby revealing a novel therapeutic target for exosome-based bone regeneration strategies.
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Registered trials
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