ArticleCell reports methods2026
Efficient CRISPR-Cas genome editing in brown algae.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Fucoidans: a new frontier in brown seaweed research and biotechnology.Cell surface (Amsterdam, Netherlands) · 2026Review
- Brown algae produce hydrocarbons via fatty acid photodecarboxylase-dependent and fatty acid photodecarboxylase-independent pathways.Plant physiology · 2026Article
- Latent endogenous giant viruses drive active infection and inheritance in a multicellular algal host.Nature microbiology · 2026Article
- Loss of canonical epigenetic silencing reveals distinct chromatin evolution in brown algae.Nature ecology & evolution · 2026Article
- Revisiting giant virus-host dynamics in brown algae: old stories and new perspectives.The EMBO journal · 2026Review
- Germline fate determination by a single ARGONAUTE protein inProceedings of the National Academy of Sciences of the United States of America · 2026Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brown algae represent the third most complex lineage to have independently evolved multicellularity, distinct from plants and animals. Yet, functional studies of their development and evolution have been limited by the absence of efficient genome editing tools. Here, we present a robust, high-efficiency, and transgene-free CRISPR-based genome editing platform applicable across four ecologically and biotechnologically important brown algal species. Using Ectocarpus as a model, we optimized a polyethylene glycol (PEG)-mediated ribonucleoprotein (RNP) delivery system that achieves reproducible editing across multiple loci without cloning or specialized equipment. As proof of concept, we recreated the hallmark imm mutant phenotype by precisely editing the IMMEDIATE UPRIGHT (IMM) locus. APT/2-fluoroadenine (2-FA) selection further enhanced specificity with minimal false positives. The method was easily transferable to other species, including kelps. This platform now enables functional genomics in brown algae, providing powerful tools for investigating development, life cycle regulation, and the independent evolution of complex multicellularity.
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Registered trials
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