ArticleMicrobiology spectrum2026
Development of a droplet digital PCR for detection and quantitation of human parvovirus B19.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Precision Dosing of Vancomycin: A Systematic Review of Population Pharmacokinetics Models and Comparative Evaluation of Software Tools.Clinical pharmacokinetics · 2026Pooled it
- Skn7 Regulates Response to Oxidative Stress in the Opportunistic Fungi Scedosporium apiospermum.Mycopathologia · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
The objective of this study is to establish a droplet digital PCR (ddPCR) method for the detection of human parvovirus B19 (B19V) and provide accurate and reliable technical support for molecular biological diagnosis and epidemiological investigation of the virus. Specific primers and a TaqMan probe targeting the NS1 region of the B19V genome were designed, and a reaction system based on ddPCR was constructed and optimized. The methodology was validated through sensitivity, specificity, and repeatability tests. Subsequently, the method was applied to eight B19V-positive samples to evaluate its practical applicability. Methodological validation experiments demonstrated excellent sensitivity with a good linear relationship ( IMPORTANCE: Early human parvovirus B19 (B19V) infections were sporadic or occurred in small clusters, attracting little attention. Since late 2023, nine European Union/European Economic Area (EU/EEA) countries have reported a significant increase in B19 infections. Moreover, the virus has robust physicochemical tolerance and the potential to resist pathogen removal processes such as filtration, inactivation, and pasteurization, which have raised the close attention and vigilance of international organizations, governments, and the public. Despite existing qPCR and antigen/antibody tests, the growing number of infections in multiple countries highlights the need for a more accurate and efficient detection system. Based on this, our team carried out related research and built a system configuration based on the third-generation droplet digital polymerase chain reaction platform, with a view to updating the B19V detection method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.