ArticleJournal of clinical microbiology2026
Challenges in accurate HDV RNA quantification: inter-assay variability and the impact of thermal shock.
Felipe Pérez-García, Ana Virseda-Berdices, Carlos Pita-Martínez, Mario Muñoz Monte, Daniel Sepúlveda-Crespo, Helena Codina, Roberto Alonso, Lara Mesones, Sandra Rodrigo, Juan Macías and 4 more
Abstract readEvaluation StudyComparative Study
In one paragraphArticle in Journal of clinical microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
14 authors.
Felipe Pérez-GarcíaServicio de Microbiología Clínica, Hospital Universitario Príncipe de Asturias, Madrid, Spain.ORCID 0000-0002-4885-4334 Ana Virseda-BerdicesCentro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.
Carlos Pita-MartínezUnidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
Mario Muñoz MonteServicio de Microbiología Clínica, Hospital Universitario Príncipe de Asturias, Madrid, Spain.
Daniel Sepúlveda-CrespoCentro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.ORCID 0000-0002-8053-6045 Helena CodinaCentro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.
Roberto AlonsoServicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.ORCID 0000-0002-0516-6371 Lara MesonesServicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Sandra RodrigoServicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Juan MacíasCentro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.
Luis Miguel RealCentro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.ORCID 0000-0003-4932-7429 Juan Cuadros-GonzálezServicio de Microbiología Clínica, Hospital Universitario Príncipe de Asturias, Madrid, Spain.
Isidoro Martínez *Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.ORCID 0000-0002-9949-9264 Salvador Resino *Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.ORCID 0000-0001-8783-0450 Funding
Centro de Investigación en Red en Enfermedades Infecciosas CB21/13/00044European Society of Clinical Microbiology and Infectious Diseases ESCMIDRG17142Fundación para la Investigación Biomédica del Hospital Universitario Príncipe de Asturias VHD-HUPAInstituto de Salud Carlos III CP23CIII/00004Instituto de Salud Carlos III PI19CIII/00009Instituto de Salud Carlos III PI20CIII/00004
6 · The paper itselfAbstract
Quantitative RT-PCR (qRT-PCR) is essential for monitoring hepatitis delta virus (HDV) RNA, yet assays lack standardization. We aimed to evaluate the performance of three qRT-PCR assays and to assess the impact of a pre-analytical thermal shock procedure. We conducted a comparative study using 206 samples (106 with anti-HDV antibodies and 100 anti-HDV-negative as a control group), which were tested in parallel with three qRT-PCR assays: Vircell, Certest, and Altona. Performance was evaluated for inter-assay agreement (kappa index), quantitative correlation (R²), and bias (Bland-Altman). Altona detected 56 HDV-RNA positive samples, whereas Vircell and Certest detected 55 positive samples. Inter-assay agreement was perfect comparing Vircell vs Certest (agreement = 100%, к = 1.000) and almost perfect comparing Altona with both Vircell and Certest (agreement = 99.5%, к = 0.988). Quantitatively, Vircell and Certest assays showed relevant systematic biases compared to Altona, overestimating viral loads by approximately 0.24 log
Indexed as
Hepatitis DHepatitis Delta VirusReal-Time Polymerase Chain ReactionRNA, ViralViral LoadHumansReproducibility of ResultsSensitivity and SpecificityRNA, Viralhepatitis D virusinter-assay variabilityqRT-PCRthermal shockviral load
Identifiers
PMID41474328
PMCPMC12892981
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