Evidence map›Paper›PMID 41474163›Full record

ArticleMicrobiology spectrum2026

A two-step screen identifies a small molecule that disrupts membrane voltage and is effective against growing and persister gram-negative bacteria.

Ciara K Asamoto, Calvin A Ewing, Christian T Meyer, Samual C Allgood, Matthew J G Eldridge, Donald Evans, Toni A Nagy, Grace L Christensen, Amy L Crooks, Daqing Jiang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ciara K AsamotoDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.ORCID 0000-0003-0981-3190
Calvin A EwingDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Christian T MeyerDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.ORCID 0000-0002-8719-6522
Samual C AllgoodDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Matthew J G EldridgeDepartment of Microbiology, Harvard University, Cambridge, Massachusetts, USA.ORCID 0000-0002-6538-4012
Donald EvansDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Toni A NagyDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Grace L ChristensenDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Amy L CrooksDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Daqing JiangDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.
Sophie HelaineDepartment of Microbiology, Harvard University, Cambridge, Massachusetts, USA.ORCID 0000-0002-9877-4180
Corrella S DetweilerDepartment of Molecular, Cell, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA.ORCID 0000-0002-7783-0876

Funding

The physiological activation and consequences of Toxin-Antitoxin systems in SalmonellaR01AI155552 · NIAID · HARVARD MEDICAL SCHOOL · PI HELAINE, SOPHIE · 2021 to 2025
$2.5M
Infection-Dependent Vulnerabilities of Gram-negative Bacterial PathogensR01AI168916 · NIAID · UNIVERSITY OF COLORADO · PI Corrella S Detweiler · 2023 to 2026
$2.2M
The electrophysiology of biofilm development and drug resistanceR00AI175656 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI Christian T Meyer · 2025 to 2026
$498k
Using Salmonella Pathogenesis and Cell Biology as a Discovery ToolR21AI171945 · NIAID · UNIVERSITY OF COLORADO · PI DETWEILER, CORRELLA S · 2023 to 2024
$430k
The electrophysiology of biofilm development and drug resistanceK99AI175656 · NIAID · UNIVERSITY OF COLORADO · PI MEYER, CHRISTIAN T · 2023 to 2024
$277k
Burroughs Wellcome Fund 2022NIAID NIH HHS K99 AI175656NIAID NIH HHS R00 AI175656NIAID NIH HHS R01 AI155552NIAID NIH HHS R01 AI168916NIAID NIH HHS R21 AI171945NIH HHS K99AI175656NIH HHS R01AI155552NIH HHS R01AI168916NIH HHS R01AI168916SNIH HHS R21AI171945
6 · The paper itself

Abstract

New antibiotics effective against gram-negative bacteria are difficult to discover because many small molecules are excluded by the cell envelope. There is a growing consensus that the proton motive force (PMF) represents a key target for the development of antimicrobial agents. Here, we report data that both support that consensus and demonstrate the utility of host-based screens in discovering novel antibiotics. Previous in-host screens of small-molecule libraries have identified compounds that inhibit

Indexed as

Anti-Bacterial AgentsCell MembraneGram-Negative BacteriaSalmonella typhimuriumSmall Molecule LibrariesAnimalsHumansMacrophagesMiceMicrobial Sensitivity TestsProton-Motive ForceAnti-Bacterial AgentsSmall Molecule Librariesantibioticanti-infectivecell envelopemacrophagemembraneSalmonella Typhimurium

Identifiers

PMID41474163
PMCPMC12889115

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.