Selection and characterization of a DNA aptamer for Patulin and its application in a label-free fluorescence assay for fruit juices.

Sihan Wang, Jiayi Liang, Haiyang Jiang, Jianzhong Shen, Zhanhui Wang, Juewen Liu

Abstract read

In one paragraph

Article in One health advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sihan WangDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1 Canada.

Jiayi LiangDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1 Canada.

Haiyang JiangDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.

Jianzhong ShenDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.

Zhanhui WangDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.

Juewen LiuDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1 Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Patulin (PTL, 154 Da) is a small molecule toxin produced as a mold metabolite. PTL is unstable in animals and is quickly converted to its metabolites. Thus, few successful antibodies were reported even after extensive efforts in hapten design. In this work, a library immobilization strategy was used to obtain DNA aptamers for PTL, where exposure of PTL to live animals or cells is no longer needed. After 20 rounds of selection, the most abundant aptamer named PTL-1 has a dissociation constant ( Graphical Abstract: Supplementary Information: The online version contains supplementary material available at 10.1186/s44280-025-00101-2.

Indexed as

AptamersBiosensorsFruit juicePatulinSELEX

Identifiers

PMID41473783

DOI10.1186/s44280-025-00101-2

PMCPMC12745320

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.