ArticleTransboundary and emerging diseases2025
Development of an Indirect ELISA Based on the PEDV S Protein and Evaluation of Its Correlation With Neutralizing Antibody and Immune Protection.
Article in Transboundary and emerging diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- A ferritin nanoparticle-displayed spike protein-based ELISA for serological surveillance and immune evaluation of porcine deltacoronavirus.Virologica Sinica · 2026Article
- Development of an automated chemiluminescence immunoassay for detection of IgA antibodies against porcine epidemic diarrhea virus spike.BMC veterinary research · 2026Article
- Development of an Indirect ELISA Based on the PEDV S Protein and Evaluation of Its Correlation With Neutralizing Antibody and Immune Protection.Transboundary and emerging diseases · 2025Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that infects pigs of all ages. Notably, neonatal piglets (≤7 days old) in naïve herds can experience case fatality rates approaching 100%, with hallmark clinical signs of vomiting, diarrhea, and dehydration, thereby representing a major threat to swine herd health. Serum PEDV-specific IgG serves as a key serological marker of vaccine-induced herd immunity. Accordingly, we developed an indirect ELISA (iELISA) using Chinese hamster ovary (CHO) cell-expressed recombinant PEDV spike (S) protein as the coating antigen. Checkerboard titration identified the optimal antigen-coating concentration and serum dilution as 1 μg/mL and 1:300, respectively. Based on sample-to-positive (S/P) ratios from 50 PEDV-negative sera, the diagnostic cutoff was set at 0.796, corresponding to the mean plus 3 standard deviation (SD) of the negative control group (
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