Evidence map›Paper›PMID 41473108›Full record

ArticleFrontiers in veterinary science2025

Development of a novel p72 gene-based loop-mediated isothermal amplification assay for the rapid detection of African swine fever virus in animal feed.

Beilei Ge, Kelly J Domesle, Janine A Simmons, Shenia R Young, David A Brake, Ryan C McDonald, Haile F Yancy, Lindsay R Gabbert, John G Neilan, Chris A Whitehouse

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Development and validation of a rapidFrontiers in microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Beilei GeU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.
Kelly J DomesleU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.
Janine A SimmonsSAIC, Plum Island Animal Disease Center, Greenport, NY, United States.
Shenia R YoungU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.
David A BrakeBioQuest Associates, LLC, Stowe, VT, United States.
Ryan C McDonaldU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.
Haile F YancyU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.
Lindsay R GabbertU.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY, United States.
John G NeilanU.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY, United States.
Chris A WhitehouseU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Applied Science, Laurel, MD, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Rapid and reliable detection of the African swine fever virus (ASFV), the causative agent of often fatal African swine fever, in animal feed is critical for implementing timely emergency control measures. Methods: We developed a novel loop-mediated isothermal amplification (LAMP) assay targeting the ASFV p72 gene ( Results: LAMP1 was the fastest, with positive results obtained in as early as 3.8 min [compared to at least 5.5 min for LAMP2 and 20 cycles for real-time PCRs (~15 min)]. These assays could detect ASFV from 10 Discussion: Further validation of this novel p72 gene-based LAMP assay in animal feed will pave the way for its adoption as a rapid screening tool in ASFV feed surveillance, as well as potential application in outbreak response and recovery efforts, to safeguard the nation's animal feed supply.

Indexed as

African swine fever virusanimal feeddetectionloop-mediated isothermal amplificationmajor capsid protein

Identifiers

PMID41473108
PMCPMC12745283

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.