Evidence map›Paper›PMID 41473078›Full record

ArticleJTCVS open2025

A custom preservation solution extends the ex vivo availability of living heart valves for transplantation.

Elizabeth M Cordoves, Baoqi Liu, Olivier Pont, V Reed LaSala, Griffin Welsh Daly, Keith Yeager, Lucie Chicaud, Richard Zhuang, Senay Ustunel, Kavya Rajesh and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JTCVS open, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Elizabeth M CordovesVagelos College of Physicians and Surgeons, Columbia University, New York, NY.
Baoqi LiuDepartment of Biomedical Engineering, Columbia University, New York, NY.
Olivier PontDepartment of Surgery, Columbia University, New York, NY.
V Reed LaSalaDepartment of Surgery, Columbia University, New York, NY.
Griffin Welsh DalyDepartment of Biomedical Engineering, Columbia University, New York, NY.
Keith YeagerDepartment of Biomedical Engineering, Columbia University, New York, NY.
Lucie ChicaudDepartment of Surgery, Columbia University, New York, NY.
Richard ZhuangDepartment of Biomedical Engineering, Columbia University, New York, NY.
Senay UstunelDepartment of Surgery, Columbia University, New York, NY.
Kavya RajeshVagelos College of Physicians and Surgeons, Columbia University, New York, NY.
Nicole JuliaDepartment of Biomedical Engineering, Columbia University, New York, NY.
Sophia JackmanDepartment of Surgery, Columbia University, New York, NY.
Sharon FleischerDepartment of Biomedical Engineering, Columbia University, New York, NY.
Emile A BachaSection of Congenital and Pediatric Cardiac Surgery, Division of Cardiac, Thoracic, and Vascular Surgery, NY-Presbyterian Morgan Stanley Children's Hospital, Columbia University Medical Center, New York, NY.
Emmanuel ZornColumbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.
Giovanni FerrariDepartment of Biomedical Engineering, Columbia University, New York, NY.
Gordana Vunjak-NovakovicDepartment of Biomedical Engineering, Columbia University, New York, NY.
David M KalfaColumbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.

Funding

Engineering a strategy for the preservation and rehabilitation of living allogenic heart valvesF30HL174098 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Elizabeth Marie Cordoves · 2024 to 2026
$161k
NHLBI NIH HHS F30 HL174098
6 · The paper itself

Abstract

Objectives: Although heart valve transplantation delivers living, growth-capable valves, its clinical implementation is restricted by transplant logistics and donor availability. We developed a preservation solution for the ex vivo storage of living valves that maintains tissue viability for at least 7 weeks. Methods: Porcine pulmonary roots (N = 25) were dissected, treated with a clinically used antibiotic cocktail, and stored in a custom-made preservation solution (Valve Preservation Solution, VPS) or Hanks' Balanced Salt Solution with serum for up to 7 weeks. Tissues were preserved in normothermia (37 °C, 5% CO Results: Cold-stored valves (VPS and Hanks' Balanced Salt Solution) demonstrated significantly reduced viability within 1 to 2 weeks ex vivo. In contrast, normothermic storage in VPS preserved leaflet viability for 7 weeks, with consistent glucose metabolism. Immunostaining of normothermic VPS-stored valve leaflets showed a quiescent cell phenotype with little expression of alpha-smooth muscle actin or proliferation markers (relative to baseline), and baseline-level leaflet Vimentin expression. Despite preserved cellular viability, leaflet microarchitectural integrity was only maintained for 3 weeks of ex vivo storage. Conclusions: Normothermic preservation of living valves can support tissue viability for at least 7 weeks ex vivo. With additional preclinical validation, stored living valves could act as a tissue source for heart valve transplantation, with key advantages in enhanced availability and resource-efficiency.

Indexed as

biobankheart valve transplantliving valvepartial heart transplantvalve preservation

Identifiers

PMID41473078
PMCPMC12745111

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.