A fully automated model to form "dry surface biofilms" under optimal dehydration conditions. application to Enterobacteriaceae in healthcare settings.

Nicolas Jean-Marie, Talyssa Lebielle, Myriam Louisin, Claude Olive, Karine Marion-Sanchez

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Article in Biofilm, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Nicolas Jean-MarieCHU Martinique, Hygiene and Environment Laboratory, Fort-de-France, Martinique.

Talyssa LebielleCHU Martinique, Hygiene and Environment Laboratory, Fort-de-France, Martinique.

Myriam LouisinCHU Martinique, Hygiene and Environment Laboratory, Fort-de-France, Martinique.

Claude OliveCHU Martinique, Hygiene and Environment Laboratory, Fort-de-France, Martinique.

Karine Marion-SanchezCHU Martinique, Hygiene and Environment Laboratory, Fort-de-France, Martinique.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Over ten years ago, bacteria attached to surfaces and surrounded by extracellular polymeric substances were observed on dry surfaces in intensive care units. These structures were named "dry surface biofilms" (DSBs). Most in vitro models used to study "DSBs" alternate long hydration phases with short periods of desiccation, producing "semi-dehydrated DSBs" that differ from the conditions in healthcare settings. Our aim was to create a model that could produce "DSBs" under optimal dehydration conditions and apply it to Enterobacteriaceae. These bacteria are commonly responsible for healthcare-associated infections in our hospital, yet they have received little attention in the context of "DSBs." We developed a fully automated model that mimics the splashing of respiratory secretions by repeatedly nebulizing an inoculum of contaminated artificial saliva. Hydration phases lasted 2 s every 6 h. We investigated the microscopic aspect, mean surface coverage, bacterial culturability and membrane integrity. After validating the model with methicillin-resistant

Indexed as

dried inoculumDry surface biofilmEnterobacteriaceaeHealthcare settingMethicillin- resistant Staphylococcus aureusModelXDR Klebsiella pneumoniae

Identifiers

PMID41472835

DOI10.1016/j.bioflm.2025.100312

PMCPMC12745990

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.