ArticleFrontiers in microbiology2025
Growth-coupled selection of 5-aminolevulinic acid synthetase mutants for 5-aminolevulinic acid biosynthesis in
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: 5-Aminolevulinic acid (5-ALA) is a nonprotein amino acid with broad applications in agriculture, medicine, and the food industry. In recent years, substantial efforts have been devoted to enhancing its biosynthesis. The catalytic activity of 5-aminolevulinic acid synthase (ALAS) is a critical determinant of production efficiency. Methods: In this study, a growth-coupled selection strategy was developed to improve ALAS from Results: The enzymatic activity of the best mutant, D4,7,18, increased by 67.41%, leading to 1.18-fold higher 5-ALA accumulation than that of the wild type. Enzymatic analysis suggested that the enhanced activity of D4,7,18 was partly attributable to stronger PLP binding and a lower Conclusion: The growth-coupled selection strategy demonstrated in this study offers a promising approach for optimizing other enzymes and metabolic pathways, provided that an appropriate selection strain and screening conditions are employed.
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