ArticleVeterinary world2025
Sperm freezability is associated with melatonin receptor expression in the sperm plasma membrane of Saanen goat bucks
Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors.
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Abstract
Background and Aim: Cryopreservation is an essential assisted reproductive technology for preserving valuable animal genetics; however, it induces osmotic and oxidative stress that compromises sperm quality. Melatonin (MLT) functions as both an antioxidant and a signaling molecule through specific membrane receptors, melatonin 1 (MT1) and melatonin 2 (MT2). This study aimed to determine the association between sperm freezability, MLT receptor expression on spermatozoa, and MLT concentration in the seminal plasma of goat bucks. Materials and Methods: Semen samples were collected weekly from seven clinically healthy Saanen bucks (2-3 years) maintained under natural photoperiod and standardized feeding conditions. A total of 124 ejaculates were analyzed seasonally (spring-winter). Sperm quality parameters, including motility, viability, capacitation status (chlortetracycline [CTC] assay), plasma membrane fluidity, and acrosome integrity, were evaluated before and after cryopreservation. Expression levels of MT1 and MT2 receptors were quantified by Western blot, while MLT concentration in seminal plasma was measured by enzyme-linked immunosorbent assay. Pearson's correlation and determination coefficients (R, R Results: Western blot analysis revealed variable MT1 (16 kDa) and MT2 (28-75 kDa) expression throughout the year. High negative correlations (R > -0.9, R Conclusion: Sperm freezability in goats is closely linked to the expression of MLT receptors and seminal plasma MLT concentration. Higher receptor expression corresponds to improved post-thaw sperm quality, likely by mitigating cryocapacitation and acrosomal damage. These findings suggest that enhancing MLT receptor expression or modulating photoperiodic exposure could optimize semen cryopreservation protocols and reproductive efficiency in caprine species.
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