Evidence map›Paper›PMID 41472250›Full record

ArticleViruses2025

Development and Application of a Rapid Field Detection Technology for DENV-2 Based on the HUDSON Nucleic Acid Extraction-Free/RT-RAA/CRISPR-Cas12a System.

Chang Tan, Siyu Xing, Xiaoxue Xie, Xiaoli Chen, Xiaohui Liu, Wenhao Wang, Lifang Liu, Xinyu Zhou, Jiahong Wu, Chunxiao Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chang TanCharacteristic Key Laboratory of Modern Pathogen Biology, School of Basic Medicine Sciences, Guizhou Medical University, Guian New Area, Guiyang 561113, China.
Siyu XingState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Xiaoxue XieState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Xiaoli ChenState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.ORCID 0009-0009-8047-2473
Xiaohui LiuState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Wenhao WangState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Lifang LiuState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Xinyu ZhouState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Jiahong WuCharacteristic Key Laboratory of Modern Pathogen Biology, School of Basic Medicine Sciences, Guizhou Medical University, Guian New Area, Guiyang 561113, China.
Chunxiao LiCharacteristic Key Laboratory of Modern Pathogen Biology, School of Basic Medicine Sciences, Guizhou Medical University, Guian New Area, Guiyang 561113, China.

Funding

Chunxiao-Li 2024YFC2607800
6 · The paper itself

Abstract

Dengue fever has become a major global public health challenge due to its rapidly in-creasing incidence. Rapid on-site detection of dengue virus (DENV) is critical for early diagnosis, timely patient isolation, and outbreak control. In this study, dengue virus serotype 2 (DENV-2), the predominant strain circulating in tropical and subtropical regions, was selected as the target pathogen. We established a one-tube rapid detection assay that integrates the HUDSON nucleic acid extraction-free protocol, reverse transcription recombinase-aided amplification (RT-RAA), and CRISPR/Cas12a-mediated trans cleavage activity. The method achieved a detection limit of 1 × 10

Indexed as

CRISPR-Cas SystemsDengueDengue VirusNucleic Acid Amplification TechniquesAnimalsBacterial ProteinsCRISPR-Associated ProteinsEndodeoxyribonucleasesHumansRNA, ViralSensitivity and SpecificityBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesRNA, ViralCRISPR/Cas12aDENV-2detectionHUDSONRT-RAA

Identifiers

PMID41472250
PMCPMC12737410

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.