Evidence map›Paper›PMID 41472240›Full record

ArticleViruses2025

The Interaction Network of NSm and Its Role as a Movement Protein in the Tomato Zonate Spot Virus.

Xingyue Zhao, Jianbin Chen, Limin Zheng, Jiajia Tu, Xin Wang, Xiaobin Shi, Yu Zhang, Shue Sun, Jie Zhang, Xue Zheng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xingyue ZhaoHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Jianbin ChenHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Limin ZhengMicroscopy Core Facility, The Biomedical Research Core Facility, Westlake University, Hangzhou 310024, China.
Jiajia TuGeneral Equipment & Autoclave Service Core Facility, The Biomedical Research Core Facility, Westlake University, Hangzhou 310024, China.
Xin WangHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Xiaobin ShiHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Yu ZhangHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Shue SunHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Jie ZhangYunnan Province Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China.
Xue ZhengYunnan Province Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China.
Deyong ZhangHunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.

Funding

Agriculture Research System of China No. CARS-23-D-02Changsha Outstanding Innovative Youth Training Program kq2406020National Natural Science Foundation of China Nos. 32302300National Natural Science Foundation of Hunan Province No.2025JJ60138 and No.2025JJ60137
6 · The paper itself

Abstract

The tomato zonate spot virus (TZSV) poses a significant threat to agriculture. Therefore, the elucidation of the functional roles and interactions of its encoded proteins is crucial for the development of effective control strategies. The aim of this study was to investigate the interaction network between the TZSV nucleocapsid (N), the non-structural M-segment (NSm) and the non-structural S-segment (NSs) proteins, with a focus on the functional characterization of the NSm protein. Yeast two-hybrid (Y2H) analysis indicated that both the N protein (N-N) and the NSm protein (NSm-NSm) exhibit self-interaction in vitro, with successful expression of all fusion proteins confirmed by Western blotting. Subsequently, we used bimolecular fluorescence complementation (BiFC) and luciferase complementation imaging (LCI) assays in epidermal cells of

Indexed as

FlexiviridaePlant DiseasesPlant Viral Movement ProteinsSolanum lycopersicumViral Nonstructural ProteinsNicotianaNucleocapsid ProteinsProtein BindingTwo-Hybrid System TechniquesNucleocapsid ProteinsPlant Viral Movement ProteinsViral Nonstructural Proteinsco-localizationmovement proteinprotein–protein interactionsself-interactionsubcellular localizationtomato zonate spot virus

Identifiers

PMID41472240
PMCPMC12737781

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.