Evidence map›Paper›PMID 41471253›Full record

ArticlePathogens (Basel, Switzerland)2025

Development of a Novel Sandwich ELISA Test for the Detection of Antibodies Against Rickettsia.

Marco Quevedo-Diaz, Semen Kaliukanov, Frantisek Csicsay, Diana Hopkova, Ludovit Skultety

Abstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marco Quevedo-DiazDepartment of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.ORCID 0009-0006-0011-3680
Semen KaliukanovDepartment of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.
Frantisek CsicsayDepartment of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.ORCID 0009-0005-8845-5855
Diana HopkovaDepartment of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.ORCID 0000-0002-7515-6734
Ludovit SkultetyDepartment of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.ORCID 0000-0002-9830-6459

Funding

Scientific Grant Agency of the Ministry of Education and Slovakian Academy of Sciences VEGA 2/0129/24Scientific Grant Agency of the Ministry of Education and Slovakian Academy of Sciences VEGA 2/0147/24the Recovery and Resilience Plan of the Slovak Republic 09I03-03-V04-00556the Slovakian Research and Development Agency APVV-23-0325
6 · The paper itself

Abstract

Diagnosis of rickettsial infections is challenging due to nonspecific clinical symptoms and limitations of current diagnostic methods. Molecular assays allow early detection but are limited by cost and technical demands, whereas conventional serological tests often exhibit cross-reactivity and low sensitivity during the early stages of infection. This study aimed to develop and evaluate a recombinant-antigen sandwich ELISA for improved antibody detection against

Indexed as

Antibodies, BacterialRickettsiaRickettsia InfectionsAnimalsAntigens, BacterialCross ReactionsEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin GImmunoglobulin MRecombinant ProteinsReproducibility of ResultsSensitivity and SpecificityAntibodies, BacterialAntigens, BacterialImmunoglobulin GImmunoglobulin MRecombinant ProteinsA8GP63DnaKGroELrecombinant proteinRickettsia akaririckettsial infectionsandwich ELISA

Identifiers

PMID41471253
PMCPMC12736059

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.