Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan.

Hirohisa Mekata, Mari Yamamoto, Aya Matsuu, Ken Maeda, Haruhiko Isawa, Kentaro Yoshii, Kazumi Umeki, Tamaki Okabayashi

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Serological and molecular assessment of severe fever with thrombocytopenia syndrome virus infection in wild boars from two adjacent endemic prefectures in Japan.The Journal of veterinary medical science · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hirohisa MekataCenter for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki City 889-2192, Japan.ORCID 0000-0003-3063-0997

Mari YamamotoCenter for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki City 889-2192, Japan.

Aya MatsuuCollege of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-0880, Japan.ORCID 0009-0004-9493-9613

Ken MaedaDepartment of Veterinary Science, National Institute of Infectious Diseases, Japan Institute for Health Security, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.ORCID 0000-0002-3488-5439

Haruhiko IsawaDepartment of Medical Entomology, National Institute of Infectious Diseases, Japan Institute for Health Security, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.

Kentaro YoshiiDepartment of Viral Ecology, National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University, 1-12-4 Sakamoto, Nagasaki City 852-8523, Japan.ORCID 0000-0002-1104-0898

Kazumi UmekiDepartment of Internal Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake 889-1692, Japan.ORCID 0000-0003-4392-8127

Tamaki OkabayashiCenter for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki City 889-2192, Japan.ORCID 0000-0001-8614-6593

Funding

Japan Agency for Medical Research and Development JP256f0137007Japan Agency for Medical Research and Development JP25fk0108717Japan Society for the Promotion of Science 23K21273Japan Society for the Promotion of Science 25K22364

6 · The paper itself

Abstract

Oz virus, an emerging tick-borne thogotovirus, has been reported to cause fatal human infection in Japan. However, its ecology and geographic distribution remain largely unknown. In this study, we developed a double-antigen sandwich enzyme-linked immunosorbent assay (DAgS ELISA) for detecting Oz virus antibodies in animals and used it to conduct a seroepidemiological survey of wild boars (

Indexed as

Antibodies, ViralSus scrofaSwine DiseasesThogotovirusAnimalsAntigens, ViralEnzyme-Linked Immunosorbent AssayFemaleJapanMaleSensitivity and SpecificitySeroepidemiologic StudiesSwineAntibodies, ViralAntigens, Viraldouble-antigen sandwich ELISAOz virusseroepidemiological surveyThogotoviruswild boar

Identifiers

PMID41471243

DOI10.3390/pathogens14121288

PMCPMC12735430

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.