Evidence map›Paper›PMID 41471186›Full record

ArticlePathogens (Basel, Switzerland)2025

Development of a TaqMan-Based Duplex Real-Time Quantitative RT-PCR for Detection and Differentiation of Muscovy Duck Reovirus and Novel Duck Reovirus.

Li Liu, Jinping Fu, Mengzhou Lin, Anping Wang, Shuang Wu, Chuanmin Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Li LiuSchool of Veterinary Medicine and Public Health, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Jinping FuInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID 0000-0002-5120-1174
Mengzhou LinCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Anping WangSchool of Veterinary Medicine and Public Health, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.ORCID 0000-0002-6288-8936
Shuang WuSchool of Veterinary Medicine and Public Health, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Chuanmin LiuInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

Funding

Jiangsu Academy of Agricultural Sciences Scientific Research Fund Project KYJJ(25)0704Jiangsu Agri-Animal Husbandry Vocational College Key Technology Innovation Funding Project for Industrial Development NSF2022ZR02Jiangsu Agri-Animal Husbandry Vocational College Science and Technology Innovation Team Program NSF2023TC02Jiangsu Agriculture Science and Technology Innovation Fund CX(24)3002Jiangsu University "Qinglan Project" Su Jiaoshi Han (2020) No. 10Nanjing Science and Technology Innovation Funding Project for Overseas Researchers 025083501230360National Undergraduate Training Program for Innovation and Entrepreneurship X2025104521131Shandong Provincial Natural Science Foundation, China ZR2020MC019Shandong Provincial Natural Science Foundation, China ZR2021LZY030
6 · The paper itself

Abstract

Muscovy duck reovirus (MDRV) and novel duck reovirus (NDRV) are major pathogens in duck breeding, leading to substantial economic losses in the waterfowl industry. This study aimed to develop a precise detection and differentiation method for both viruses simultaneously. Specific primers and probes targeting the S3 gene were designed, and a duplex TaqMan-based real-time RT-PCR assay was established following optimization of reaction conditions. The assay demonstrated high amplification efficiency (100.1-106.7%), strong linear correlation (R

Indexed as

Orthoreovirus, AvianPoultry DiseasesReal-Time Polymerase Chain ReactionReoviridae InfectionsReverse Transcriptase Polymerase Chain ReactionAnimalsDucksReproducibility of ResultsSensitivity and Specificitymuscovy duck reovirus (MDRV)novel duck reovirus (NDRV)real-time PCRTaqMan probe

Identifiers

PMID41471186
PMCPMC12735753

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.