Evidence map›Paper›PMID 41471166›Full record

ArticlePathogens (Basel, Switzerland)2025

HPV16 E6 and E7 Genetic Variability in Oral and Anal Samples from HIV-Positive MSM.

Verdiana Zulian, Silvia Pauciullo, Roberta Sciamanna, Antonio Coppola, Martina De Sanctis, Arianna Borsellino, Paola Del Porto, Franca Del Nonno, Daniele Colombo, Alessandra Scarabello and 1 more

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Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Verdiana ZulianLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.ORCID 0009-0005-8996-4803
Silvia PauciulloLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.ORCID 0009-0009-7188-7132
Roberta SciamannaLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.
Antonio CoppolaLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.
Martina De SanctisLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.ORCID 0009-0009-2628-6880
Arianna BorsellinoDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00100 Rome, Italy.
Paola Del PortoDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00100 Rome, Italy.
Franca Del NonnoPathology Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.ORCID 0000-0001-6664-9257
Daniele ColomboPathology Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.
Alessandra ScarabelloClinical and Research Infectious Diseases Department, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.
Anna Rosa GarbugliaLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), 00149 Rome, Italy.ORCID 0000-0002-2687-8642

Funding

Ministero della Salute Ricerca corrente Linea 2
6 · The paper itself

Abstract

Persistent infection with high-risk human papillomavirus (HPV), particularly genotype 16, is the main driver of anogenital and oropharyngeal cancers. However, data on HPV16 genetic variability in anal and oral samples from people living with HIV are limited. In this study, we investigated the diversity of HPV16 E6 and E7 genes in anal and oral samples collected from HIV-positive men who have sex with men (MSM) according to different cytological outcomes and anatomical site. Among 53 MSM patients, we obtained 51 E6 and 52 E7 sequences. Lineage A predominated (96.1%), mainly represented by sublineages A1 (84.3%) and A2 (9.8%). The E6 region showed higher variability than E7: 31.4% were identical to the reference (K02718), 51.0% carried the L83V (T350G) mutation, while Q14H, D25N, and D25E were also observed. In E7, only one non-synonymous substitution was detected (N29S). No site-specific variants were detected between anal and oral samples. Overall, these findings confirm the predominance of European A1/A2 variants in Italian MSM and suggest that E6/E7 polymorphisms are not directly linked to lesion grade or anatomical localization.

Indexed as

Anal CanalGenetic VariationHIV InfectionsHuman papillomavirus 16MouthOncogene Proteins, ViralPapillomavirus E7 ProteinsPapillomavirus InfectionsRepressor ProteinsAdultGenotypeHomosexuality, MaleHumansMaleMiddle AgedE6 protein, Human papillomavirus type 16oncogene protein E7, Human papillomavirus type 16Oncogene Proteins, ViralPapillomavirus E7 ProteinsRepressor ProteinsE6 proteinE7 proteinHPV16human papillomavirusvariant

Identifiers

PMID41471166
PMCPMC12736272

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.